| 角色 | 饰演者 | 角色介绍 |
| Boss Baby | 亚历克·鲍德温 | |
| Tim | 迈尔斯·克里斯托弗.. | |
| Dad | 吉米·坎摩尔 | |
| Mom | 莉莎·库卓 | |
| Adult Tim / Narrator | 托比·马奎尔 | |
| Francis Francis | 史蒂夫·布西密 | |
| Staci | ViviAnn Yee | |
| Triplets | Eric Bell Jr. | |
| Wizzie / Elvis Impersonator | James McGrath | |
| Eugene | 康拉德·弗农 | |
| Jimbo | 大卫·索伦 | |
| Big Boss Baby | Edie Mirman | |
| Story Bear | James Ryan | |
| Photographer | 沃尔特·道恩 | |
| Crying Boy / Little Boy / Boy | Jules Winter | |
| Tims Daughter / Girl / Little Girl | Nina Zoe Bakshi | |
| TV Chef | 汤姆·麦克格雷斯 | |
| Airport Security Guard | Brian Hopkins | |
| Airport Announcer | Glenn Harmon | |
| Elvis Impersonators | Joseph Izzo | |
| Captain Ross | 克里斯·米勒 | |
| Flight Attendant | Andrea Montana Knoll | |
| Hazmat Baby | Chloe Albrecht |
