| 角色 | 饰演者 | 角色介绍 |
| Sam Witwicky | 希亚·拉博夫 | |
| Carly | 罗茜·汉丁顿-惠特莉 | |
| Major William Lennox | 乔什·杜哈明 | |
| Robert Epps | 泰瑞斯·吉布森 | |
| Megatron (voice) | 雨果·维文 | |
| Sentinel Prime (voice) (rumored) | 伦纳德·尼莫伊 | |
| Dylan | 帕特里克·德姆西 | |
| Bruce | 约翰·马尔科维奇 | |
| Marissa Faireborn | 弗兰西斯·麦克多蒙德 | |
| Dutch | 艾伦·图代克  | |
| Simmons | 约翰·特托罗 | |
| Soundwave (voice) | 弗兰克·维尔克 | |
| Wheelie (voice) | 汤姆·肯尼 | |
| Optimus Prime (voice) | 彼特·库伦 | |
| Silverbolt (voice) | James Avery | |
| Mearing's Aide | Keiko Agena | |
| Ron Witwicky | 凯文·杜恩 | |
| Judy Witwicky | 朱丽·怀特 | |
| Ratchet (voice) | 罗伯特·福克沃斯 | |
| Jessica Morgan | Kym Whitley | |
| Hardcore Eddie | Mighty Rasta | |
| General Morshower | 格伦·莫肖尔 | |
| Starscream (voice) (as Charlie Andler) | Charles Adler | |
| (voice) | 马克·瑞安 | |
| Ironhide (voice) | 杰斯·哈梅尔 | |
| Lt. Sulemani | Anthony Azizi | |
| Russian | Elena Kolpachikova | |
| Stevens | Kevin Sizemore | |
| Russian Bartender | Kathleen Gati | |
| General Morshowers Aide | Lamonica Garret | |
| NASA scientist | David St. James | |
| Berated NASA Scientist | Darren O'Hare | |
| Jack | Jack Donner | |
| NASA Scientist | Lindsey Ginter | |
| President | 布雷特·史泰利 | |
| Pimply Executive | Chris Sheffield | |
| USAF Tech Sergeant Allen | Scott Levy | |
| Executive Interviewer | Michael Loeffelholz |
