| 角色 | 饰演者 | 角色介绍 |
| 迪亚戈（中文配音） | 黄磊 | |
| 海狸（中文配音） | 黄忆慈 | |
| Manny | 雷·罗马诺 | |
| Sid | 约翰·雷吉扎莫  | |
| Diego | 丹尼斯·利瑞 | |
| Crash | 西恩·威廉·斯科特 | |
| Eddie | 乔希·佩克 | |
| Ellie | 奎恩·拉提法 | |
| Lone Gunslinger Vulture | 威尔·阿奈特 | |
| Fast Tony | 杰·雷诺 | |
| Scrat | 克里斯·韦奇 | |
| Dung Beetle Dad | Peter Ackerman | |
| Glypto Boy Billy / Beaver Girl | Caitlin Rose Ander.. | |
| Rhino Boy / Beaver Boy | Connor Anderson | |
| Mr. Start | Joseph Bologna | |
| Elk Boy | Jack Crocicchia | |
| Condor Chick | 彼得·德塞弗 | |
| Start Girl | Nicole DeFelice | |
| Diatryma Mom | 德比·戴瑞巴里 | |
| Start Dad | Marshall Efron | |
| Stu / Male Ox | Tom Fahn | |
| Molehog Grandpa / Shovelmouth Male | Jason Fricchione | |
| Traffic Vulture | James Edmund Godwin | |
| Beaver Dad | George Jacobs | |
| Female Mini Sloth / Dung Beetle Mom | Clea Lewis | |
| Glyptodon | 布莱恩·麦克法登 | |
| Shovelmouth Boy | 詹森·潘妮蒂尔 | |
| Start Boy 1 | Gregory Romano | |
| Start Boy 2 | Matthew Romano | |
| Aardvark Dad | 斯蒂芬·鲁特 | |
| Dodo | 卡洛斯·沙尔丹哈 | |
| Molehog Kid 1 | Manoela Scarpa Sal.. | |
| Molehog Kid 2 | Sofia Scarpa Salda.. | |
| Diegos Bird Girl | Reyna Shaskan | |
| Freaky Male | James Sie | |
| Female Ox | Mindy Sterling | |
| James | Alex Sullivan | |
| Mrs. Start | Renée Taylor | |
| Cholly | 艾伦·图代克  | |
| Additional Voices | Emily Anderson | |
| Additional Voices | 杰克·安琪 | |
| Additional Voices | Shane Baumel | |
| Additional Voices | Bob Bergen | |
| Additional Voices | Claudia Besso | |
| Additional Voices | Kyle J. Bogert | |
| Additional Voices | Rodger Bumpass | |
| Additional Voices | 保罗·布切 | |
| Additional Voices | Blanca Camacho | |
| Additional Voices | John Cygan | |
| Additional Voices | Jennifer Darling | |
| Additional Voices | Olivia DeLaurentis | |
| Additional Voices | Harrison Fahn | |
| Additional Voices | 比尔·法默 | |
| Additional Voices | Crispin Freeman | |
| Additional Voices | Spencer Lacey Ganus | |
| Additional Voices | 杰斯·哈梅尔 | |
| Additional Voices | Tim Kirkpatrick | |
| Additional Voices | David H. Kramer | |
| Additional Voices | Sherry Lynn | |
| Additional Voices | 丹尼·曼 | |
| Additional Voices | 劳拉·马兰洛 | |
| Additional Voices | Madeleine Martin | |
| Additional Voices | Matthew P. McCarthy | |
| Additional Voices | 米凯·麦高万 | |
| Additional Voices | Laraine Newman | |
| Additional Voices | Jan Rabson | |
| Additional Voices | 扎克·沙达 | |
| Additional Voices | Thomas Sharkey | |
| Additional Voices | Ross Simanteris | |
| Additional Voices | Rachel Diaz-Stand | |
| Additional Voices | 阿芮尔·温特 | |
| Aardvark Mom | Cindy Slattery |
