| 角色 | 饰演者 | 角色介绍 |
| Mario Mario | 鲍勃·霍斯金斯 | |
| Luigi Mario | 约翰·雷吉扎莫  | |
| King Koopa | 丹尼斯·霍珀 | |
| Princess Daisy | 萨曼莎·玛西丝 | |
| Iggy | 费舍·史蒂芬斯 | |
| Spike | Richard Edson | |
| Lena | 菲奥娜·肖 | |
| Daniella | Dana Kaminski | |
| Toad | Mojo Nixon | |
| Scapelli | Gianni Russo | |
| Bertha | Francesca Roberts | |
| The King | 兰斯·亨利克森 | |
| Old Lady | Sylvia Harman | |
| Angelica | Desiree Marie Velez | |
| Brooklyn Girl | 安德瑞·鲍威尔 | |
| Brooklyn Girl | Heather Pendergast | |
| Brooklyn Girl | Melanie Salvatore | |
| Goomba Toad | John Fifer | |
| Sergeant Simon | Don Lake | |
| Hatcheck Girl | Terry Finn | |
| Goomba | Thomas Merdis | |
| Goomba | Michael Harding | |
| Goomba | Michael Lynch | |
| Goomba | Scott Mactavish | |
| Goomba | Wallace Merck | |
| Nun | Mona B. Fierro | |
| Nun | Karen Brigman | |
| Nun | Christi Work | |
| Nun | Joy Rees | |
| Nun | Lucy Alpaugh | |
| TV Announcer | Robert D. Raiford | |
| Reporter #1 | 哈里·墨菲 | |
| Reporter #2 | Patt Noday | |
| Scapelli Bodyguard | Robert Lee Edwards | |
| Scapelli Bodyguard | Ronald Lou Edwards | |
| Pizza Deliveryman | Matthew Zachary Hopkins | |
| Egan (as Robert Faulkner Priester) | Robert Priester | |
| James | Preston Lane | |
| Japanese Businessman #1 | Jim Asaki | |
| Japanese Businessman #2 | Matt Nikko | |
| Devo Controller | Kevin West |
