| Peter Parker? (13 episodes 1999-2001) | Rino Romano | |
| Machine Men? (12 episodes 1999-2001) | Dale Wilson | |
| Dr. Naoko Yamada-Jones (10 episodes 1999-2001) | Akiko Morison | |
| High Evolutionary? (10 episodes 1999-2001) | Richard Newman | |
| John Jameson (10 episodes 1999-2001) | John Payne | |
| Sir Ram? (9 episodes 1999-2001) | Ron Halder | |
| Shane Yamada-Jones (9 episodes 1999-2001) | Rhys Huber | |
| Eddie Brock? (8 episodes 1999-2001) | Brian Drummond | |
| Karen O'Malley (8 episodes 1999-2001) | Kimberly Hawthorne | |
| Carnage? (7 episodes 1999-2001) | Michael Donovan
 | |
| Bromley? (7 episodes 1999-2001) | Christopher Gaze | |
| Additional Voices? (7 episodes 1999-2001) | Scott McNeil | |
| Lady Ursa (7 episodes 1999-2001) | Tasha Simms | |
| Lord Tyger (7 episodes 1999-2001) | David Sobolov
 | |
| Lady Vermin? (6 episodes 1999-2001) | Jennifer Hale | |
| Additional Voices? (5 episodes 1999-2001) | 凯思林·巴尔
 | |
| Mr. Meugniot? (4 episodes 1999-2001) | Gary Chalk | |
| Additional Voices? (2 episodes 1999-2001) | Paul Dobson | |
| Additional Voices (2 episodes 2001) | Janyce Jaud | |
