| Samanosuke Akechi (voice) | Robin Atkin Downes | |
| Jacques Blanc (French) (voice) | 让·雷诺
 | |
| Jacques Blanc (English) (voice) | Paul Mercier | |
| Ako (voice) | Amber Hood | |
| Michelle Aubert (English) (voice) | Jennifer Hale | |
| Henri Blanc (English) (voice) (as BJ Ward) | B.J. Ward | |
| Nobunaga Oda (voice) | Richard Doyle | |
| Ranmaru Mori (voice) | Joshua Keaton | |
| Guildenstern (voice) | Neil Ross | |
| Vega Donna (voice) | Vanessa Marshall | |
| Tadakatsu Heihachiro Honda (Heihachi)/Gargant (voice) (as Dee Baker) | Dee Bradley Baker | |
| Mitsuhide Akechi (voice) | Paul Eiding | |
| Hideyoshi Hashiba (voice) | Armin Shimerman | |
| Soldier (voice) | Daran Norris | |
| Merchant (voice) | Bart Flynn | |
| Worker (voice) | Ben Judd | |
| Spirit of Onimusha (voice) | Dorian Harewood
 | |
| Voice of Mother (voice) | 裘蒂·班森
 | |
| (voice) | Odile Cohen | |
| (voice) (as Jacky Berger) | Jackie Berger | |
| (voice) (as Maurice Decoster) | Maurice De Coster | |
| (voice) | Roland Timsit | |
| (voice) (as Jean Loup Horwitz) | Jean-Loup Horwitz | |
| (voice) | Pierre Forest | |
| (voice) (as Johana Menuteau) | Johanna Menuteau | |
| (voice) | Nathalie Homs | |
| (voice) | Soase Le Bras | |
