| 角色 | 饰演者 | 角色介绍 |
| Dr. Gregory House (158 episodes 2004-2011) | 休·劳瑞 | |
| Dr. James Wilson (157 episodes 2004-2011) | 罗伯特·肖恩·莱纳德 | |
| Dr. Eric Foreman (156 episodes 2004-2011) | 欧玛·艾普斯 | |
| Dr. Robert Chase (156 episodes 2004-2011) | 杰西·斯宾塞 | |
| Dr. Remy 'Thirteen' Hadley? (79 episodes 2007-2011) | 奥利维亚·王尔德 | |
| Nurse? (112 episodes 2005-2011) | Bobbin Bergstrom | |
| Dr. Lawrence Kutner (36 episodes 2007-2009) | 卡尔·潘 | |
| Dr. Amber Volakis (18 episodes 2007-2009) | 安妮·达德克 | |
| Nurse Brenda Previn (11 episodes 2005-2006) | Stephanie Venditto | |
| Stacy Warner (9 episodes 2005-2006) | 雪拉·渥德 | |
| Dr. Jeffrey 'Big Love' Cole (7 episodes 2007) | 艾迪·盖瑟吉 | |
| Michael Tritter (6 episodes 2006-2007) | 大卫·摩斯 | |
| Rachel Taub (15 episodes 2008-2011) | Jennifer Crystal | |
| Hospital Pharmacist (6 episodes 2004-2007) | Marco Pelaez | |
| Dr. Simpson (7 episodes 2005-2011) | Ron Perkins | |
| Edward Vogler (5 episodes 2005) | 切·麦克布瑞德 | |
| Dr. Travis Brennan (5 episodes 2007) | Andy Comeau | |
| Mark Warner (4 episodes 2005-2006) | 库里·格拉汉姆 | |
| Dr. Chen Anesthesiologist? (4 episodes 2005-2007) | Hira Ambrosino |
