| 角色 | 饰演者 | 角色介绍 |
| Selene | 凯特·贝金赛尔 | |
| Eve | 英迪娅·埃斯利 | |
| Quint | Kristen Holden-Reid | |
| Jacob | 斯蒂芬·瑞 | |
| David | 提奥·詹姆斯 | |
| Lida | 桑德琳·霍尔特 | |
| Lycan #1 | Richard Cetrone | |
| Officer Kolb | 雅各布·布莱尔 | |
| British Newscaster | Sanny Van Heteren | |
| Troop leader | Kurt Max Runte | |
| Lab Worker #3 (uncredited) | Daniel Boileau | |
| Vampire #1 (uncredited) | Jesse Branden Dahl | |
| Desk Guard (uncredited) | Lee Majdoub | |
| Young Scientist (uncredited) | Tyler McClendon | |
| Old City Cop #1 (uncredited) | 帕努 | |
| Lycan Creature 2 (uncredited) | 丹·佩恩 | |
| Alan (uncredited) | Adam Reid | |
| Guard (uncredited) | Christian Tessier | |
| Announcing Guard | Mark Gibbon | |
| Surgical Nurse | Benita Ha | |
| (uncredited) | 韦斯·本特利 | |
| Olivia | Catlin Adams | |
| Old City Cop #2 | Ian Rozylo | |
| Cadillac Driver (uncredited) | Joe Censoplano | |
| Medical Supervisor | John Innes | |
| Antigen Surgeon (uncredited) | Preston Cook |
