| 角色 | 饰演者 | 角色介绍 |
| Agent J | 威尔·史密斯 | |
| Young Agent K | 乔什·布洛林 | |
| Agent K | 汤米·李·琼斯 | |
| Boris The Animal | 杰梅奈·克莱门特 | |
| Agent O | 艾玛·汤普森 | |
| Griffin | 迈克尔·斯图巴 | |
| Boris' Girlfriend | 尼可·斯彻金格 | |
| Colonel | Mike Colter | |
| Jeffrey Price | Michael Chernus | |
| Young Agent O | 爱丽丝·伊芙 | |
| Agent X | 大卫·拉斯彻 | |
| Mr. Wu | Keone Young | |
| Andy Warhol | 比尔·哈德尔 | |
| Colonel's Son | Cayen Martin | |
| Prison Guard #1 | Clarke Thorell | |
| Prison Guard #2 | Adam Mucci | |
| Prison Guard #3 | Tom McComas | |
| Prison Guard #4 | Douglas Crosby | |
| 2012 MIB HQ Guard | Woodie King Jr. | |
| 1969 MIB HQ Guard | Jack O'Connell | |
| 1969 MIB Agent #1 | Tobias Segal | |
| 1969 MIB Agent #2 | Jon Shaver | |
| 1969 MIB Agent #3 | Gerritt Vandermeer | |
| Mom | Alexandra O'Hara | |
| Little Chocolate Milk Girl | Violet O'Hara | |
| Coney Island Hippie | Valence Thomas | |
| Coney Island Flower Child | Chloe Sonnenfeld | |
| Obadiah Price | Lanny Flaherty | |
| MIB Desk Agent | Jonathan O'Hara | |
| Brain Alien | 里克·贝克 | |
| New York Mets Fan #1 | Joseph D'onofrio | |
| New York Mets Fan #2 | 约瑟夫·甘纳斯考利 | |
| Diner Waitress | Katy Frame | |
| Funky '60s Dude | Kevin Townley | |
| Guru | Stephen Brian Jones | |
| Muscle Boy at Happening | Tyler Johnson |
