| 角色 | 饰演者 | 角色介绍 |
| Alucard (voice) | 中田让治 | |
| Sir Integra Fairbrook Wingates Hellsing (voice) | 榊原良子 | |
| Seras 'Police Girl' Victoria (voice) | 折笠富美子 | |
| Walter 'Angel of Death' C. Dornez (voice) | 清川元梦 | |
| Father Alexander Anderson (voice) | 若本规夫 | |
| Pip Bernadotte (voice) | 平田广明 | |
| Bishop/Archbishop Enrico Maxwell (voice) | 田中秀幸 | |
| The Major/SS-Sturmbannführer (voice) | 飞田展男 | |
| Jan Valentine (voice) | 中井和哉 | |
| Luke Valentine (voice) | 子安武人 | |
| Richard Hellsing (voice) | 秋元羊介 | |
| Enrico Maxwell/Priest (voice: English version) | JB Blanc |
