| 角色 | 饰演者 | 角色介绍 |
| Miss Bianca (voice) | 伊娃·嘉宝 | |
| Wilbur (voice) | 约翰·坎迪 | |
| Percival C. McLeach (voice) | 乔治·C·斯科特 | |
| Joanna and additional special vocal effects (voice) | 弗兰克·维尔克 | |
| Red (voice) | 彼得·弗斯 | |
| Nurse Mouse (voice) | 露西·泰勒 | |
| Frank (voice) | Wayne Robson | |
| Krebbs (voice) | Douglas Seale | |
| Chairman/Doctor (voice) | Bernard Fox | |
| Baitmouse (voice) | Billy Barty | |
| Francois (voice) | Ed Gilbert | |
| Faloo/Mother (voice) | Carla Meyer | |
| Radio Announcer/Airplane Captain (voice) (uncredited) | Peter Greenwood | |
| Bernard (voice) | 鲍勃·纽哈特 | |
| Jake (voice) | Tristan Rogers |
