| 角色 | 饰演者 | 角色介绍 |
| Kenshin Himura (voice) (as Richard Hayworth) | Richard Cansino | |
| Kaoru Kamiya / Kuro (voice) (as Dorothy Melendrez) | Dorothy Melendrez | |
| Sanosuke Sagara (voice) | Alexis Lang | |
| Yahiko Myojin / Yumi Komagata (voice) (as Elyse Floyd) | Wendee Lee | |
| Megumi Takani (voice) | Jane Alan | |
| Tae / Tsubame (voice) (as Georgette Rose) | Michelle Ruff | |
| Dr. Gensai / Jirokichi Ebisu / Shibumi / Sugimoto (voice) | Steve Kramer | |
| Suzame / Soshi Okita (voice) | Mona Marshall | |
| Ayame (voice) | Steve Bloch | |
| Sakata / Additional Voices (voice) | Robert Axelrod | |
| Yohei Senbonya (voice: English version) | Milton James | |
| Gohei / Senaku (voice) (as John Billingslea) | Beau Billingslea | |
| Makoto Shishio / Toma Sakaki (voice) (as David Lucas) | Steven Blum | |
| Additional Voices (voice) | Bob Bobson | |
| Kanryu Takeda (voice) | Ivan Buckley | |
| Additional Voices (voice) | Francis Cherry | |
| Additional Voices (voice) | Peter Doyle | |
| Shikijo (voice: English version) (as Dean Elliot) | Dean Elliott | |
| Raijuta Isurugi / Hiko Seijuro XIII (voice) (as Richard George) | Richard Epcar |
