| Marcus Superbus | 弗雷德里克·马奇
 | |
| Empress Poppaea | 克劳黛·考尔白
 | |
| Emperor Nero | 查尔斯·劳顿
 | |
| Mercia | Elissa Landi | |
| Tigellinus | Ian Keith | |
| Titus | Arthur Hohl | |
| Favius | Harry Beresford | |
| Stephan | Tom Conlon | |
| Glabrio | Ferdinand Gottschalk | |
| Dacia | Vivian Tobin | |
| Licinius | William V. Mong | |
| Ancaria (as Joyzelle) | Joyzelle Joyner | |
| Viturius | Richard Alexander | |
| Strabo | Nat Pendleton | |
| Servillius | Clarence Burton | |
| Tybul | Harold Healy | |
| Philodemus (as Robert Manning) | Robert Manning | |
| Tyros | Charles Middleton | |
| Bombadier - 1944 Re-Release Prologue (uncredited) | Joel Allen | |
| Christian in Dungeon (uncredited) | 米沙·奥尔
 | |
| Bettor of 300 Silver (uncredited) | Lionel Belmore | |
| Slave of Nero (uncredited) | True Boardman | |
| Roman Woman (uncredited) | Marjorie Bonner | |
| Mute Giant (uncredited) | Joe Bonomo | |
| Spectator at Colosseum (uncredited) | Henry Brandon | |
| Slave of Nero (uncredited) | George Bruggeman | |
| (uncredited) | Horace B. Carpenter | |
| Christian Martyr Saying "Victory" / Roman citizen outside of Colisseum / Gladiator saying 'We who are about to die.' (uncredited) | 约翰·卡拉丁 | |
