| Alex (voice) | 本·斯蒂勒
 | |
| Gloria (voice) | 贾达·萍克·史密斯
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| Marty (voice) | 克里斯·洛克
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| Melman (voice) | 大卫·休默
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| Gia (voice) | 杰西卡·查斯坦
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| Vitaly (voice) | 布莱恩·科兰斯顿
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| King Julien XIII (voice) | 萨莎·拜伦·科恩
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| Captain Chantel DuBois (voice) | 弗兰西斯·麦克多蒙德
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| Stefano (voice) | 马丁·肖特
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| Mort (voice) | 安迪·里查克
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| Maurice (voice) | 塞德里克·凯尔斯
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| Skipper (voice) | 汤姆·麦克格雷斯
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| Kowalski (voice) | 克里斯·米勒
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| Private (voice) | Christopher Knights | |
| Carmen | 帕兹·维嘉
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| Animals' vocal effects (voice) (uncredited) | 弗兰克·维尔克
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| Mason / Second Policeman (voice) | 康拉德·弗农
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| Freddie the Dog (voice) | 维尼·琼斯
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| Jonesy the Dog (voice) | Steve Jones | |
| Frankie the Dog (voice) | Nick Fletcher | |
| Croupier / Circus Master (voice) | 丹尼·雅各布
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| Casino Security / Mayor of New York City (voice) | Dan O'Connor | |
| Comandante / Zoo Official / Zoo Announcer (voice) | 埃里克·达尼尔
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| Fourth Policeman (voice) | Stephen Kearin | |
| Zoo Official (voice) | Emily Nordwind | |
