| 角色 | 饰演者 | 角色介绍 |
| Ai Haibara (voice) | 林原惠美 | |
| Suzu Chiga (voice) | Mari Adachi | |
| Inspector Megure (voice) | 茶风林 | |
| Heiji Hattori (voice) | 堀川亮 | |
| Enkai (voice) | Keisuke Ihara | |
| Inspector Shiratori (voice) | 井上和彦 | |
| Ayumi Yoshida (voice) | 岩居由希子 | |
| Seizo Sakura (voice) | Sabur? Kamei | |
| Kogoro Mori (voice) | 神谷明 | |
| Sonoko Susuki (voice) | 松井菜樱子 | |
| Kazuha Toyama (voice) | 宫村优子 | |
| Ryuhen (voice) | 中村大树 | |
| Doctor Agatha (voice) | 绪方贤一 | |
| Fumimaro Ayanokoji (voice) | 置鲇龙太郎 | |
| Mitsuhiko Tsuburaya (voice) | 大谷育江 | |
| Tae Yamakura (voice) | Hiroko Suzuki | |
| Taiga Saijo (voice) | 铃置洋孝 | |
| Genta Kojima (voice) | 高木涉 | |
| Shinichi Kudo (voice) | 山口胜平 | |
| Harutaro Mizuo (voice) | 游佐浩二 |
