| 角色 | 饰演者 | 角色介绍 |
| Gil Grissom | 威廉·彼德森 | |
| Catherine Willows | 玛格·海根柏格 | |
| Warrick Brown | 加里·杜尔丹 | |
| Nick Stokes | 乔治·艾德斯 | |
| Sara Sidle | 乔雅·福克斯 | |
| Captain Jim Brass | 保罗·盖尔福勒 | |
| Eddie Willows | Timothy Carhart | |
| Officer Joe Tyner | Tony Crane | |
| Teri Miller | 帕米拉·吉德利 | |
| Dr. Al Robbins | 罗伯特·大卫·豪尔 | |
| Amy Hendler | K.K. Dodds | |
| Jason Hendler | Andy Buckley | |
| Greg Sanders | 艾瑞克·兹曼达 | |
| Mrs. Green | Anne E. Curry | |
| Ted Beaton | Michael Delano | |
| April Lewis | Roxanne Day | |
| Al | Leonard Kelly-Young | |
| Detective B. Evans | Mark Collver | |
| Bobby Dawson | Gerald McCullouch | |
| Lindsey Willows | Madison McReynolds | |
| Valet (as Phillip Jean Marie) | Phillip Jeanmarie |
