| Alice | 米拉·乔沃维奇Milla Jovovich | |
| Claire Redfield | 艾丽·拉特Ali Larter | |
| Chris Redfield | 文特沃斯·米勒Wentworth Miller | |
| Bennett | 金·寇兹
 | |
| Albert Wesker | 肖恩·罗伯茨Shawn Roberts | |
| Angel Ortiz | 塞尔乔·佩里斯-曼切塔Sergio Peris-Mencheta | |
| K-Mart | 史宾塞·洛克Spencer Locke | |
| Luther West | 波瑞斯·科乔Boris Kodjoe | |
| Jill Valentine | 西耶娜·盖尔利Sienna Guillory | |
| Crystal | 凯茜·巴恩费尔德Kacey Barnfield | |
| Kim Yong | Norman Yeung | |
| Wendell | Fulvio Cecere | |
| Axeman | Ray Olubowale | |
| Sniper#1 | Christopher Kano | |
| Sniper#2 | Tatsuya Goke | |
| Technician#1 | Nobuya Shimamoto | |
| Duty Officer | Peter Kosaka
 | |
| Captain Hotaka | Dennis Akayama | |
| Technician#2 | Kenta Tomeoki | |
| Umbrella Sergeant | Shin Kawai | |
| J Pop Girl | 中岛美嘉
 | |
| Business Man | Takato Yamashita | |
| Kogel Girl Undead | Hiromi Okuyama | |
| Featured Zombie(uncredited) | Bill Blair | |
| Zombie(uncredited) | Evin Michaels；Devon Richards；Valerie Ruescas | |
