| Slutpira | Izzie Harlow | |
| Trash (segment "Slay Ride") | Ruby Larocca | |
| Dr. Schoal (segment "One Foot in the Grave") | George Stover | |
| Lucia (segment "Blood Witch") | Countess Samela | |
| Derek (segment "Mike Wuz Here") | Josh Lively | |
| Juan Carlos (segment "Blood Witch") | Kert Rats | |
| Juan Domingo (segment "Blood Witch") | Grim Parsons | |
| Isabella / Blood Witch (segment "Blood Witch") | Monica Puller | |
| Juana / Carla (segment "Blood Witch") | Cassandra Complex | |
| Kelly (segment "Blood Witch") | Priscilla Lee Press-On | |
| Mrs. Kellysworth (segment "Blood Witch") | Amanda Garcia | |
| Telemarketer (segment "Blood Witch") | Floyd Pimpton | |
| Bug Man (segment "Blood Witch") | D.A. Meadows | |
| Mike (segment "Blood Witch") | T.J. Rogers | |
| Alex (segment "Blood Witch") | Zack Boyce | |
| Gene (segment "Mike Wuz Here") | Chris Rhodes | |
| Lydia (segment "Mike Wuz Here") | Heather Lucas | |
| Gretchen (segment "Mike Wuz Here") | Ashley Goddard | |
| Jerry (segment "Mike Wuz Here") | Eric Carlson | |
| Greg (segment "Mike Wuz Here") | Jordan Hess | |
| Frank (segment "Mike Wuz Here") | Steve McElroy | |
