| 角色 | 饰演者 | 角色介绍 |
| Fox Mulder (175?episodes 1993-2002) | 大卫·杜楚尼 | |
| Dana Scully?/ ... (196?episodes 1993-2002) | 吉莲·安德森 | |
| John Doggett (40?episodes 2000-2002) | 罗伯特·帕特里克 | |
| Walter Skinner?/ ... (82?episodes 1994-2002) | 米彻·佩勒吉 | |
| Monica Reyes?/ ... (23?episodes 2001-2002) | 安娜贝丝·吉什 | |
| CGB Spender?/ ... (37?episodes 1993-2002) | William B. Davis | |
| Melvin Frohike (39?episodes 1994-2002) | Tom Braidwood | |
| John Fitzgerald Byers (36?episodes 1994-2002) | 布鲁斯·哈伍德 | |
| Richard 'Ringo' Langly?/ ... (35?episodes 1994-2002) | Dean Haglund | |
| Alex Krycek?/ ... (24?episodes 1994-2002) | Nicholas Lea | |
| FBI Deputy Director Alvin Kersh?/ ... (19?episodes 1998-2002) | James Pickens Jr. | |
| Margaret Scully (16?episodes 1994-2002) | Sheila Larken | |
| Elder #1?/ ... (14?episodes 1995-1999) | Don S. Williams | |
| Mr. X (14?episodes 1994-2002) | Steven Williams | |
| FBI Special Agent Jeffrey Spender?/ ... (13?episodes 1996-2002) | Chris Owens | |
| Deep Throat (11?episodes 1993-1999) | Jerry Hardin | |
| Mrs. Teena Mulder (10?episodes 1995-2000) | Rebecca Toolan | |
| Marita Covarrubias (10?episodes 1996-2002) | 劳瑞·侯登 |
