| 角色 | 饰演者 | 角色介绍 |
| Dr. Baron Victor Frankenstein | 彼得·库欣 | |
| Anna Spengler | 维罗妮卡·卡尔森 | |
| Professor Richter | 弗雷迪·琼斯 | |
| Dr. Karl Holst | 西蒙·沃德 | |
| Inspector Frisch | Thorley Walters | |
| Ella Brandt | 玛克辛·奥德丽 | |
| Dr. Frederick Brandt | George Pravda | |
| Police Doctor | Geoffrey Bayldon | |
| Mad Woman | Colette O'Neil | |
| Third Houseguest (plumber) | Frank Middlemass | |
| Fourth Houseguest (playing chess) | George Belbin | |
| Second Houseguest (smoking pipe) | Norman Shelley | |
| First Houseguest (reading newspaper) | Michael Gover | |
| Asylum Principal | Peter Copley | |
| Dr. Otto Heideke | Jim Collier | |
| Police Sergeant | Allan Surtees | |
| Sergeant Brenner | Windsor Davies | |
| Burglar | Harold Goodwin | |
| Policeman (uncredited) | Timothy Davies | |
| Official (uncredited) | Robert Davis | |
| Passer-By (uncredited) | Caron Gardner | |
| Mortuary Attendant (uncredited) | Robert Gillespie | |
| Warder (uncredited) | Michael Goldie | |
| Water Board Workman (uncredited) | Edward Higgins | |
| Christina Ella's Friend (uncredited) | Elizabeth Morgan | |
| Lady In Garden (uncredited) | Daphne Oxenford | |
| Anna's Neighbour (uncredited) | Dorothy Smith |
