| Peter Parker? (65 episodes 1994-1998) | Christopher Daniel Barnes | |
| Kingpin? (33 episodes 1995-1998) | Roscoe Lee Browne
 | |
| Mary Jane Watson? (32 episodes 1995-1998) | Sara Ballantine | |
| Joe 'Robbie' Robertson? (27 episodes 1994-1998) | Rodney Saulsberry | |
| Felicia Hardy? (27 episodes 1995-1997) | Jennifer Hale | |
| J. Jonah Jameson (26 episodes 1994-1998) | 爱德华·阿斯纳
 | |
| Harry Osborn? (23 episodes 1995-1998) | Gary Imhoff | |
| Aunt May Parker (19 episodes 1994-1997) | Linda Gary | |
| Alistair Smythe? (18 episodes 1995-1998) | 麦克西维尔·考菲德
 | |
| Dr. Curt Connors? (17 episodes 1994-1997) | Joseph Campanella
 | |
| Michael Morbius? (15 episodes 1995-1997) | Nick Jameson | |
| Norman Osborn? (14 episodes 1995-1998) | Neil Ross | |
| Debra Whitman (13 episodes 1994-1997) | Liz Georges | |
| Herman Schultz? (12 episodes 1995-1997) | 吉姆·库宁斯
 | |
| Lt. Terri Lee? (12 episodes 1995-1997) | Dawnn Lewis | |
| Herbert Landon? (12 episodes 1995-1997) | 大卫·沃纳
 | |
| Anna Watson (12 episodes 1996-1998) | 玛耶尔·巴莱特
 | |
| Flash Thompson (11 episodes 1995-1998) | Patrick Labyorteaux | |
