| 角色 | 饰演者 | 角色介绍 |
| John Wilkes Booth | 罗伯·莫洛 | |
| President Abraham Lincoln | 兰斯·亨利克森 | |
| Mary Todd Lincoln | 唐娜·墨菲 | |
| Lucy Hale | Jean Louisa Kelly | |
| Robert Todd Lincoln | 威尔·惠顿 | |
| Lewis Thornton Paine | 提图斯·维里沃 | |
| David E. Herold | 杰姆·沃尔福特 | |
| Frederick Seward | 杰瑞米·西斯托 | |
| George A. Atzerodt (as Kirk B. R. Woller) | Kirk B.R. Woller | |
| Secretary of State William Henry Seward | John Pleshette | |
| Mr. Crook | 格里高利·伊齐恩 | |
| Thomas 'Tad' Lincoln | 亚当·兰伯格 | |
| Gen. Ulysses Simpson Grant | 约翰·阿什顿 | |
| Secretary of War Edwin McMasters Stanton | Eddie Jones | |
| Mary E. Surratt | Nancy Robinette | |
| Doctor Leale | Doug Olear | |
| Major Rathbone | Sean Baldwin | |
| Clara Harris | Mercedes Herrero | |
| John Harrison Surratt | Jason Bowcutt | |
| Mr. Parker | Marty Lodge | |
| James T. Ford | Tom Quinn | |
| Lieutenant | Michael Noel | |
| Doctor Samuel Alexander Mudd | Gary Wheeler | |
| Burke Booth's Photographer | Bob Supan | |
| Gardner Lincoln's Photographer | Tim Carlin | |
| Senator Henry's Wife | Martha Thimmesch | |
| Ned Spangler (as Dan DePaola) | Dan De Paola | |
| John George Nicolay | Scott Rinker | |
| Lieutenant Doherty | Bob Child | |
| Harry Hawk | Kevin Murray | |
| Laura Keene | O'Mara Leary |
