| John Wilkes Booth | 罗伯·莫洛
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| President Abraham Lincoln | 兰斯·亨利克森
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| Mary Todd Lincoln | 唐娜·墨菲
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| Lucy Hale | Jean Louisa Kelly | |
| Robert Todd Lincoln | 威尔·惠顿
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| Lewis Thornton Paine | 提图斯·维里沃
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| David E. Herold | 杰姆·沃尔福特
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| Frederick Seward | 杰瑞米·西斯托
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| George A. Atzerodt (as Kirk B. R. Woller) | Kirk B.R. Woller
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| Secretary of State William Henry Seward | John Pleshette | |
| Mr. Crook | 格里高利·伊齐恩
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| Thomas 'Tad' Lincoln | 亚当·兰伯格
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| Gen. Ulysses Simpson Grant | 约翰·阿什顿
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| Secretary of War Edwin McMasters Stanton | Eddie Jones
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| Mary E. Surratt | Nancy Robinette | |
| Doctor Leale | Doug Olear
 | |
| Major Rathbone | Sean Baldwin | |
| Clara Harris | Mercedes Herrero | |
| John Harrison Surratt | Jason Bowcutt | |
| Mr. Parker | Marty Lodge | |
| James T. Ford | Tom Quinn | |
| Lieutenant | Michael Noel | |
| Doctor Samuel Alexander Mudd | Gary Wheeler | |
| Burke Booth's Photographer | Bob Supan | |
| Gardner Lincoln's Photographer | Tim Carlin | |
| Senator Henry's Wife | Martha Thimmesch | |
| Ned Spangler (as Dan DePaola) | Dan De Paola | |
| John George Nicolay | Scott Rinker | |
| Lieutenant Doherty | Bob Child | |
| Harry Hawk | Kevin Murray | |
| Laura Keene | O'Mara Leary | |
